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(Pericytes, Endothelial cells, Astrocytes, Smooth muscle cells (iSMCs),…
Pericytes
DMEM/GlutaMAX (Thermo Fisher, 10566016) with
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On days 3 - 5PEL2 medium (STEMCELL, cat. no. 05270)
200 ng ml−1 of VEGF (Peprotech, cat. no. 100-20)
2 µM forskolin (Peprotech, cat. no. 6652995)
DMEM/GlutaMAX (Thermo Fisher, 10566016)
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Dulbecco’s modified Eagle’s medium (DMEM)/F12, Thermo Fisher Scientific, cat. no. 11320033, for 1 h at 37 °C)
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days 1–2, Mesoderm Induction Medium (STEMCELL, cat. no. 05220)
Endothelial cells
on days 3-5 APEL2 medium (STEMCELL, cat. no. 05270) with
200 ng ml−1 of VEGF (Peprotech, cat. no. 100-20)
2 µM forskolin (Peprotech, cat. no. 6652995)
for next 5 days StemFlex Medium (Thermo Fisher Scientific, catalog no. A3349401).(SF medium) with
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EC medium (ECM; PromoCell, cat. no. C-22011)
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on days 1-2 Mesoderm Induction Medium (STEMCELL, cat. no. 05220)
Dulbecco’s modified Eagle’s medium (DMEM)/F12, Thermo Fisher Scientific, cat. no. 11320033,) for one hour
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IPS cells
Essential 8 Flex Medium (E8F; Thermo Fisher Scientific, cat. no. A2858501)
Integrated medium
VASC medium
two-thirds ECM without supplement (PromoCell, cat. no. C-22011)
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50 ng ml−1 of VEGF (Peprotech, cat. no. 100-20) for the first 4 days