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Vatine, G. D. et al. Human iPSC-Derived Blood-Brain Barrier Chips Enable…

- - - - Human Endothelial Serum-Free Medium (hESFM; Thermo Fisher)
      - 1% Human Platelet-Poor Plasma-derived bovine Serum (PDS; Alfa Aesar)
      - 20 ng/mL basic Fibroblast Growth Factor (bFGF)
      - 1% Penicillin-Streptomycin
      - Continuous microfluidic flow at 60 uL/h (shear stress ~0.3 dyn/cm2)
  - - - 500 mL Basal Astrocyte Medium
        
        2% FBS (10 mL)
        
        1% Astrocyte Growth Supplement (AGS; 5 mL)
        
        1% Penicillin/Streptomycin (5 mL)
- - - - DMEM/F-12 basal
      - 20% KnockOut Serum Replacement (KSR)
      - 1% MEM Non-Essential Amino Acids (NEAA)
      - 1 mM L-glutamine (GlutaMAX)
      - 0.1 mM beta-mercaptoethanol
      - Daily medium change
  - - - Human Endothelial-SFM
      - 20 ng/mL bFGF
      - 10 uM all-trans Retinoic Acid