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Kumarasamy, M. & Sosnik, A. Heterocellular spheroids of the…

- - - - ECM Basal Medium
      - 5% FBS (yielding 2.5% final concentration)
      - 1% Endothelial Cell Growth Supplement (ECGS)
      - 1% Penicillin-Streptomycin
  - - - Neurobasal-A Basal Medium
      - 2% B-27 Supplement (yielding 1% final concentration)
      - 0.5 mM GlutaMAX
      - 1% Penicillin-Streptomycin
- - - - Ice-cold Hank's Balanced Salt Solution (HBSS)
      - 0.25% Trypsin-EDTA
      - 100 ug/mL DNase I
      - Quenched in DMEM + 10% FBS, washed
      - Resuspended in 1:1 Integrated Media
- - - - DMEM/F-12
      - 10% FBS
      - 1% Penicillin/Streptomycin
      - Mechanical orbital shake-off at 200 rpm for 2 hours