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26 - Coggle Diagram
26
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Fluorescent Secondary Antibodies: Diluted in 5% BSA at a 1:30,000 ratio
Membrane transfer: transferred using an iBlot2 Transfer system onto nitrocellulose membranes at 23V for 4 min
Lactate Dehydrogenase Assay (LDH): 50uL of media used per reaction, w NADH measured at 450nm
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