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Gram Positive Unknown, Mannitol Salt Agar, MR Test:, (+) Acid Results:
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Mannitol Salt Agar
Possible G +:
L.l, M.l, B.c, C.x, S.e, S.a
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Positive Result:
S.a
Dextrose Fermentation
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Why test was done: Determines whether the unknown can ferment dextrose.
My result: Yellow = acid production; Unknown ferments dextrose
Next step: the positive acid result indicates the bacteria fermented dextrose and produced acidic end products. MR/VP testing used next to further characterize glucose fermentation.
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Negative Result:
L.l, M.l, B.c, C.x, S.e
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(+) Acid Results:
L.l, B.c, S.e
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MR Test:
(+) Results:
L.l, B.c, S.e
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Why test was done: Differentiates bacteria based on growth in high salt and mannitol fermentation
My result: Growth with yellow surrounding growth; Mannitol fermentation positive
Next step: The yellow color indicates acid production from mannitol fermentation, supporting S.a. Dextrose fermentation was used next.
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Why was test done: Differentiates bacteria based on the end products of glucose fermentation.
My result: MR (+) red, VP (+) red
Next step: MR positive indicate stable acidic end products were produced. VP positive indicates acetoin production. these results support the identification of S.a.
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