Please enable JavaScript.
Coggle requires JavaScript to display documents.
MICROSCOPE - Coggle Diagram
MICROSCOPE
Parts
Base
Light Source
Condenser
Diaphragm
Stage
Objective Lens
Ocular Lens
Coarse Adjustment
Fine Adjustment
Electron Microscope
Source of illumination
Accelerated electrons
Vacuum is required
Uses
Reveal details of flagella
Fimbriae
Intracellular structure of cell
Virus detection
Transmission Electron Microscope (TEM)
2D image
Internal ultrastructure
Scanning Electron Microscope (SEM)
3D image
Surface morphology
RP < 1 nm
Phase Contrast Microscope
Difference in refractive index/cell densities
Converted to high intensity
Modified condenser
Central opaque area
Transparent ring
Uses
Microbial motility
Detecting shape of living cell
Intracellular components
Fluorescence Microscope
Short wavelength
Longer wavelength
Modified with
Excitation filter
Dichromatic mirror
Barrier filter
Uses
Autofluorescence (Chlorophyll)
Microbes coated with fluorescent dye
Immunofluorescence
Properties
Good Resolution
Good Contrast
Good Magnification
Dark Field Microscope
Bright object on dark background
Modified condenser
Central opaque ring
Blocks direct light
Uses
To study living unstained cells
Thin bacteria (Spirochetes)
Key Points
Resolution Power (RP)
Unaided Human Eye → 0.2 mm
Light Microscope → 0.2 μm
Electron Microscope → 0.5 nm
Contrast
Increased by staining
Magnification
Ocular Lens (10X)
Objective Lens
4X
10X
40X
100X
Bright Field Microscope
Dark specimen on bright background
Uses
Routine examination of stained specimen
Definition
View objects that are not within resolution range of human eye