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Exercise 1: Microbial Genetics, Exercise 4: Antimicrobial Treatments, Week…

- - - - Incubate at 37 degrees Celsius for 1 week.
  - - - Plate +DNA and -DNA E. coli samples on NA plates and incubate at 37 degrees Celsius for 1 week.
- - - - Incubate at 37 degrees Celsius for 1 week.
  - - - Digest the isolated pBR322 plasmid DNA using the PstI, EcoRI, or both enzymes.
        
        Incubate the plasmid samples at 37 degrees Celsius for 60 minutes.
        
        Store the digested plasmid samples at -20 degrees Celsius for 1 week.
    - - Incubate plates at 37 degrees Celsius for 1 week.
- - - - Pick a single S. aureus colony from the NA plate and streak it for single colonies onto an NA plate.
        
        Incubate the plates at 37 degrees Celsius for 1 week.
  - - - Load 10ul of each digested plasmid sample and 5 ul of loading buffer into the wells of the agarose gel, which has since set and been submerged in the gel electrophoresis tank using TAE buffer. Also load 10ul of a 1kb DNA ladder.
        
        Run the gel at 100 volts for 60 minutes, or until the dye has run 80% of the length of the gel.
        
        Visualize the gel using a gel scanner, which illuminates the Midori Green dye using UV light.
- - - - Using sterile forceps, plate antibiotic discs onto the seeded agar.
        
        Incubate at 37 degrees Celsius for 1 week.
  - - - One participant should was their hands with soap for the duration of 'Happy Birthday' and then dry with paper towel, before pressing the same 3 fingers onto the treatment half of the respective blood agar plate.
        
        The other participant should wash their hands using alcohol-based hand sanitizer until the treatment dries, before pressing the same 3 fingers onto the treatment half of the respective blood agar plate.
        
        Incubate at 37 degrees Celsius for 1 week.
- - - - Determine the TVC of bacterial cells after treatment via autoclaving, ethanol, or moist heat.
        
        Incubate at 37 degrees Celsius for 1 week.
- - - - Identify the unknown organisms.
- - - - Incubate the plasmid samples at 37 degrees Celsius for 60 minutes.
        
        Store the digested plasmid samples at -20 degrees Celsius for 1 week.
- - - - Pick a single S. aureus colony from the NA plate and streak it for single colonies onto an NA plate.
        
        Incubate the plates at 37 degrees Celsius for 1 week.
  - - - Load 10ul of each digested plasmid sample and 5 ul of loading buffer into the wells of the agarose gel, which has since set and been submerged in the gel electrophoresis tank using TAE buffer. Also load 10ul of a 1kb DNA ladder.
        
        Run the gel at 100 volts for 60 minutes, or until the dye has run 80% of the length of the gel.
        
        Visualize the gel using a gel scanner, which illuminates the Midori Green dye using UV light.
- - - - Incubate at 37 degrees Celsius for 1 week.
- - - - incubate at 37 degrees Celsius for 1 week