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Unknown 30 have +/-, (+)E.c, (-)E.a - Coggle Diagram
Unknown 30 have +/-
MAC growth the negative
Into broth for growing
gram stain to confromation the negative only
P.a, A.f, P.v
Hydrogen Sulfur test
(-) A.f, P.a
Urease
(+)P.a
Catalase
(+)P.a
A.f(-)
Citrate
(+)A.f
(+) P.v
Indole
(+) confirm P.v
E.a, E.c
Indole
(-)E.a
M.R
(+)E.c
M.R
Into 3 TSA plate
Seperate colony into broth to get pure negative
Gram stain to confirm only positive
MSA plate
S.a, S.e (+)
mannitol salt fermentation
S.a Pink (+)
M.R
(+)S.a
V.P
(+) S.a confirm
S.e yellow (-)
M.R
(-) S.e
V.P
(+) S.e Confirm
(-) M.l, C.x, L.l, B.c
M.R
(-)M.l, C.x
Urease
(+)M.l
Catalase
(+) M.l confirm
(-)C.x
Catalase
(+) C.x confirm
(+)B.c, L.l
V.P
(+)B.c
Catalase
(+) confirm B.c
(-)L.l
Dextrose
(+) L.l confirm
(+)E.c
V.P
(-) Confirm E.c
(-)E.a
V.P
(+) Confirm E.a