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chromatography and mass spectrometry - Coggle Diagram
chromatography and mass spectrometry
introduction
chromatography separates analytes
mass spec identifies molecules by mass-to-charge ratio
commonly combined for highly sensitive analysis
HPLC
advantages
high sensitivity
accurate quantification
good resolution
limitations
expensive
requires expertise
mass spectrometry
principles
ionisation
mass analysis
detection
applications
LC-MS
toxicology
steroid analysis
advantages
very sensitivie
detects low conc.
limitations
expensive
complex instrumentation
requires skilled staff
conclusion
chromatography and MS essential in modern biochem
provide highly accurate diagnostic info
chromatography principles
phases
stationary
mobile
separation is based on chemical interactions
types
HPLC
gas chromatography
ion exchange
affinity chromatography
applications
drug analysis
steroids
HbA1c
toxicology
Evaluation
advantages
gold standard analytical methods
high diagnostic accuracy
limitations
cost
technical complexity
long setup
gas chromatography
aplications
volatile compounds
alcohol/drugs
advantages
high specificity
limitations
limited to volatile analytes