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MICROSCOPE & WITH IT'S TYPES:A Definition: Microscope is an…
MICROSCOPE & WITH IT'S TYPES:A Definition: Microscope is an instrument used to magnify and observe very small objects (like cells, tissues, bacteria) that cannot be seen with the naked eye.
FLUORESCENCE MICROSCOPE
5. Fluorescence Microscope
🔹 Description
Uses fluorescent dyes + UV light
🔹 Uses
DNA, proteins
Medical diagnosis
STEREO MICROSCOPE
3. Stereo Microscope (Dissecting Microscope)
🔹 Description
Gives 3D view
Low magnification
🔹 Magnification
10× to 50×
🔹 Uses
Insects
Small organisms
Dissection
4. Phase Contrast Microscope
🔹 Description
Used to observe living cells
Converts light differences into visible contrast
🔹 Key Feature
✔ No staining required
🔹 Uses
Cell division
Live cell study
LIGHT MICROSCOPE
1. Light Microscope (Optical Microscope)
🔹 Description
Uses visible light and glass lenses
Most common microscope in labs
🔹 Magnification
Up to 1000×
🔹 Types
Simple microscope (one lens)
Compound microscope (multiple lenses)
🔹 Uses
Observing cells, tissues, bacteria
🔹 Advantages
✔ Easy to use✔ Cheap
🔹 Disadvantages
✖ Limited resolution
ELECTRON MICROSCOPE
2. Electron Microscope
🔹 Description
Uses electron beam instead of light
Very powerful
🔹 Magnification
Up to 1,000,000×
🔹 Types
(a) TEM – Transmission Electron Microscope
Electrons pass through specimen
Shows internal structure
✔ Very detailed image✖ Specimen must be dead
(b) SEM – Scanning Electron Microscope
Scans surface of specimen
Produces 3D image
✔ Surface details✖ Expensive
CONFOCAL MICROSCOPE
6. Confocal Microscope
🔹 Description
Uses laser light
Produces very sharp 3D images
🔹 Uses
Detailed cell imaging
Research work
PHASE CONTAST Microscope
4. Phase Contrast Microscope
🔹 Description
Used to observe living cells
Converts light differences into visible contrast
🔹 Key Feature
✔ No staining required
🔹 Uses
Cell division
Live cell study
