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High Performance Liquid Chromatography - Coggle Diagram
High Performance Liquid Chromatography
2 phases
mobile is a liquid
isocratic elution
if use 1 solvent or 1 composition during the whole run
gradient elution
changes solvent or composition
changes polarity slightly
help with separation of molecules that vary in polarity
stationary phase
solid
liquid layer
ion exchange resin
microporous beads
chemically modified resin
separation mechansim
#
adsorption
partition
ion exchange
size exclusion
affinity
choosing the type of chromatography
left to right nonpolar to polar
up and down based on size of molecule
2 layers in separation
organic
water
bigger analytes are more limited to types of separation
2 general types
normal phase
mobile phase nonpolar
stationary phase is polar
reverse phase
polar mobile phase
can have several solvents
eg water followed by acetylnitrile
nonpolar (mostly) stationary phase
column is mostly nonpolar
most common column is C18
long-chain organic carbon
actually more common
system at ECU
advantages
not limited by sample volatility or thermal stability
can run a room temperature
2 interacting phases
having a liquid rather than a gas means more contact between molecules
ease of sample recovery
