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Microscopy - Coggle Diagram
Microscopy
differential stains
most common, involves 2 different colored dyes
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acid fast stain
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mycobacterium tuberculosis, mycobacterium leprae
Endospore stain
Bacillus (Bacillus anthracis) and Clostridium (clostridium tetanii, C. botulinum
sporulation/sporogenesis, germination, bacillus genera
History (1800s)
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Joseph Jackson Lister- first modern microscope, father of the surgeon Joseph Lister
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Why stains?
bacteria are colorless, small (barely visible at 1000x)
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simple stains
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basic dyes
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ex - methylene blue, crystal violet, safranin
acidic dyes
negative charge, repelled by negative charge of cell wall
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