Please enable JavaScript.

Coggle requires JavaScript to display documents.

How Genes Are Controlled, Chapter 11: How Genes Are Controlled, CHAP 12:…

- - - - Gene cloning
        
        Plasmid - small circular DNA, acts as Vector
        
        Restriction Enzymes - cut DNA at specific sequences, produce sticky ends
        
        Cuts Plasmid -> Cuts DNA -> use DNA ligase to join fragments
        
        Produce identical copies of a gene
      - Identify Genes
        
        Nucleis Acid Probe - identify specific genes in a sample
        
        Reverse Transcriptase - mRNA -> DNA, create complementary DNA
      - Gene editing - CRISPR Cas9 System -> powerful gene editing technology
      - DNA Technology in Medicine - uses for Diagnosed, Treatment, Prevent some causes
      - GMOs
        
        organism with artificially modified genes: pest resistance, improve nutrient...
        
        Risks - disrupt ecological, impact environment,..
        
        Safe - control labotary conditions,...
      - Gene therapy
        
        insert normal genes to cells, cure for genetic diseases
        
        Risks: immune system, mutate unintended
      - DNA Sequencing - break DNA -> sequence -> reassemble
      - DNA Profilling
        
        Steps: Collect -> PCR -> Gel electrophoresis -> Analyze
      - PCR
      - Gel Electrophoresis
      - Genomics - all genes Proteomics - all proteins
    - - PCR - Amplifies small DNA.
      - Gel Electropheresis -Separate DNA fragment by size