Please enable JavaScript.
Coggle requires JavaScript to display documents.
Lecture 1 - Molecular ID, Test - Coggle Diagram
Lecture 1 - Molecular ID
Tools
Markers
-
-
-
-
-
Collection?
Collect tissue, extract and amplify, DNA via PCR, sequence and analyse
Barcoding
COI/Cox1 region of mtDNA, 650 bp long (cytochrome oxidase)
Advantages
-
-
-
High interspecific var, low intraspecific var
Disadvantages
-
Too much or too little variation, specifically plants
Error risk
-
-
Low levels of sequence divergence can indicate evolution occurred recently and rapidly, natural variance may not accumulate in time
-
Terminology
-
Locus - Defined DNA sequence, may or may not be a gene
-
Haplotype - Set of alleles, unique sequence of bases compared to other haplotypes (Unique genetic segment)
-
Applications
Cryptic Species
Closely related species that are inbdistinguishable,
Fisheries
mtDNA and microsats, IDd individual pops of fish in same regi
-
-
Species
-
Biological Species Concept - Interbreeding group that is reproductively isolated from other individuals
-
Test
-
2025 - A. 1. Captive breeding programme techniques with marine ecology 2. Molecular ecology, lab tech and data analysis. 3. Haplotype network B. 1. Molecular tools in hypoxia 2. RNA purity, advantages of targeted and global RNA techniques, proteomics 3.Heat shock protein definition, change in hsp expression using figure of congeners and what species will most likely be impacted by abiotics
2024 A. 1. Applied conservation genetics 2. eDNA use plan of locations on endangered inverts and disadv and adv of eDNA
- Haplotypes
B 1. Molecular tools in abiotic effects on organisms
- Oxygen and hypoxia, mild hypoxia vs severe hypoxia talk about upregulation and RNA seq terminology
- RNA and labwork outcomes
-
2023 - A 1. Molecular ecology techniques 2. Haplotype netwrorks
- Fish ID with molecular techniques
B 1. Heat shock proteins, and global vs targeted analysis
- Quality checks of RNA, targeted vs global RNA, proteomic
- molecular techniques
2020 - A - 1. Advantages of types of markers or trools (eDNA)
2.Conservation genetics
- Haplotype network
B 1. Principles of omics technologies, provide examples
- Isolated RNA from crassostreas gigas
2019 - A 1. Conservation genetics. 2. Genetic techniques setting up protected areas
- Haplotypes (size, patterns, figures)
B 1.RNA from multiple C.gigas, look at RNA extrac
- Targeted and high throughput analysis of DNA
- Heat shock proteins and how are they controlled. Results summary
2018 - A - 1.Molecular phylogeography, 2. Genetic Techniques, 3. Phylograms
B - 1. Caption and heat shock protein expression
- Molecular tools assessing impact of changes in O2, temperature salinity in organisms
- Gene expression and quality control in PCR