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Cell Culture (BTEC 505) - Coggle Diagram
Cell Culture (BTEC 505)
Mammalian Cell Cultures
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Cell Culture Growth
Cell Culture Media (89% DMEM, 10% PBS, 1% pen/strep); sterilized using vaccuum filtration
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Growth Kinetics (seeding 6-well plates and counting triplicate wells at 5 time points to to measure growth)
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Plant Tissue Cultures
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Media Preparation
MS Medium (Murashige & Skoog): Used for general growth of callus, shoots, and explants
GC Medium (Half-strength MS + 1% sucrose + vitamins): seed germination, root growth
MS Medium (+BA + IBA) : induces organogenesis (shoot development from isolated shoot apical meristam)
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