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Protein Expression & Analysis, Protein Detection - Coggle Diagram
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Protein Detection
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Obtain the gel + soak in 1x transfer buffer+ methanol on PVDF membrane then rinse in water & equilibrate in 1x transfer buffer+ soak the sponges & filter paper in Ix transfer buffer
Insert sandwish in a tank, Use 1X transfer buffer & run at 200v for 1 hour
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Obtain the membrane, rinse & stain in Ponceau S staining to confirm transfer efficiency