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BTEC 504 - Coggle Diagram
BTEC 504
DNA
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Extraction
Proteinase K + Spin column (Quick, reliable, scalable)
Magnetic beads (Automated, High throughput)
Proteinase K + Chloroform (Pure DNA, toxic chemicals)
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Molecular Cloning (Digestion, Ligation, Transformation)
Gibson (Flexible, precise)
Golden gate (One pot, one step, scarless, efficient)
Restriction Enzyme Digestion (cheap, easy)
Protein
Visualization
2-D PAGE (Isoelectric focusing, separated by size and charge)
SDS-PAGE (Coomassie blue stain, separated by band size)
Western blotting (transfer to membrane, antibody detection by fluorescence)
Extraction
Mechanical disruption (Sonication, French press)
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Chemical detergents (SDS lysis buffer, Triton X-100 lysis buffer)
Quantification
Lowry Assay ( more steps, more sensitive)
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Bradford Assay (concentration based on absorbance, standard curve, Coomassie dye, fast and reliable)
RNA
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Quantification
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PCR
RT - PCR (Reverse transcriptase, convert RNA to cDNA)
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