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Aseptic technique - Coggle Diagram

- - - - lower temps required
      - faster death rate
      - less resistance
    - - steam under pressure at top of chamber
      - air displaced downwards
      - air trapping reduces effectiveness
      - 13 mins at 120 degrees C
    - - creates vacuum before steam introduced
      - rapid
      - 3 mins at 131 degrees C
  - - - colourless, flammable, explosive, toxic
      - destroy by alkylation
      - 21-60 degrees C
      - 40-80% humidity
      - 450-1500 mg EO/L
      - 12hrs at room temp
      - EO must be removed from chamber by aeration--> 24hrs-12 days
      - for items that can't be steam sterilised
    - - charged particles/free radicals generated in gas plasma--> disrupt metabolism of micro-organisms
      - 1hr
      - non-toxic byproducts (water and O2)
      - for items that would be damaged by steam