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BTEC 504, PCR, SDS - PAGE
(Detects protein based on size) - Coggle Diagram
BTEC 504
DNA
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Molecular Cloning
Digestion(molecular scissors) + Ligation ( joining ends) + Assembly (Combining multiple DNA fragments)
Gibson Cloning
(palindromic - NEB Builder mix, Joins Multiple DNA Fragments)
Golden Gate cloning
(non-palindromic, to be treated before assembly)
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Quantification
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PCR
(Amplification of a specific DNA sequence, this helps to check presence or absence of the DNA sequence)
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ddPCR- (Measures the amount of specific DNA or RNA in a precise and sensitive way by partitioning the mixtures into tiny droplets with mineral oil as base solution)
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Extraction
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Proteinase K digestion + spin columns (easier, robust, fragments long DNA)
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PROTEIN
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Extraction
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Physical cell lysis method (French thaw, french press, sonication
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PCR
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ddPCR- (Measures the amount of specific RNA in a precise and sensitive way by partitioning the mixtures into tiny droplets with mineral oil as base solution)
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