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Enzyme Purification - Coggle Diagram
Enzyme Purification
Techniques to obtain a purified protein (liquid chromatography)
Basics f46-47
Different systems and Bases f50-51
IEX Ion Exchange Chromatography f52-56
HIC Hydrophobic interaction chromatography f57-59
SEC Size exclusion chromatography f60-66
Affinity chromatography f67-77
Dialysis f78
Overview of purification process
Strategic approach f21-22
protein loss and balance sheet f23-25
Start and protein location f26-29
Cell disruption f29-36
Mechanically f33f
Ultrasonic disintegration f33
High pressure homogenizers /French press) f34
Bead beating f35
Chemically f36f
Enzyme treatment
Detergents f37
Protein concentration f38
Precipitation f38-41
Ultrafiltration f42-43
Characterisation and quantification
of purified protein
Overview f80
Electrophoresis f81-
Carrier matrices f83-84
Staining and quantification methods f85
Disc f86
SDS f87-88
Isoelectric Focusing IEF f89
IP determinantion f90
two-dimensional f91
Capillary CE f92
Electroosmotic flow EOF f93
western blot f94
Immunostainingf95
Spectophotometric f101
Absorption at 280 nm or 205 f101
OPA f103
Chromophores f104
Colorimetric assays f96
BIURET f98
BCA f99
BRADFORD f100
Introduction-
Protein and its purification
The isoelectric point f9
Protein basics: structure and folding f1-18
Areas of application of proteins f19