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Control of Microbial Growth - Coggle Diagram
Control of Microbial Growth
Efficacy & Mode of Action
Efficacy Factors
Number of microbes
Environment
Time of exposure
Microbial characteristics
Mode of Action
Alteration of membrane permeability
Damage to proteins
Damage to nucleic acids
Agents
Bacteriocidal
Bacteriostatic
Methods of Microbial Control
Temperature
Bacteriocidal
Autoclave
Flame/incineration
Pasteurization
Bacteriostatic
Refrigeration
Osmotic pressure
Radiation
Ionizing
Non-ionizing
Spectrum and Tests
Broad spectrum
Destroys normal flora
Narrow spectrum
More likely to affect gram positive cells
Tests
Broth dilution
Estimates MIC and MBC
Kirby-Bauer
Measures zone of inhibition
Resistance
Mechanisms
Enzymatic destruction
Prevention of penetration
Alteration of drugs target site
Rapid ejection via efflux pump
Genes
Plasmids
Transposons