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Phage Therapy, Typical-bacteriophage-structure (1), antibiotics-10-01306…
Phage Therapy
temperate
lysogenic
chronic
jumbo phage
biofilm
phage cocktails
phage cocktails
Phage Identification
Phage Kit
culturing
How do we do this
Dilutions
of what?
Lytic, Temperate, Chronic
Phages w different host ranges
Protocols?
Side Project
Rebooting?
YAC
Crcuits
Lysis
Biofilm
Gibson/golden gate assembly
hybridization
electroporate
Main Reseacrh
Phage Protocols+procedures
Jumbo Phages
Phage Cocktails
Early Stages of YAC
New Phages
Phage Anatomy
Practical Part of Project
Phage kit - learn protocol for dealing with phages
Top agar protocol to find kill zones and collect possible phages from those zones
pseudomonas fluoresces
Classifications
Domain: Bacteria
Phylum: Proteobacteria/Pseudomonadota
Class: Gammaproteobacteria
Order: Pseudomondales
Family: Pseudomonadaceae
Genus: Pseudomonas
Gram-neg
Aerobic
Lipolytic
Proteolytic
"soluble pigment called pyoverdine" -further research
Environments
Raw food
milk
sewage
diseased plants
soil
natural water
spoilage
Baseplate - identifies which bacteria will host them + decides when to trigger lysis/goal of phage
Tail Fiber - 1st point of contact between bacteria + binds receptors
Capsid - Protects DNA/Nucleic acid
Sheath - Channel for DNA to pass through into host
coiled/collapable tunnel to insert into host
Collar - structural support
Top agar protocol
