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Recombinant DNA technology 🧬 - Coggle Diagram
Recombinant DNA technology 🧬
Ligation
Join the selected DNA fragment to a cloning vector (e.g., plasmid) using DNA ligase.
Fragmentations of DNA
Break
the DNA into smaller fragments using restriction enzymes.
Purification
Purify the expressed protein or product.
Expression of recombinant gene
Allow the host organism to express the recombinant gene.
Transformation
Introduce the recombinant DNA molecule into a host organism (e.g., bacteria).
Selection of recombinant cells
Identify and select cells that have taken up the recombinant DNA molecule.
Isolation of DNA
Isolate the DNA of interest from an organism.
