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Microscopy - Coggle Diagram
Microscopy
Microscope
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Objective lens: 4 different lenses, multiplied by base 10x. Collects light from specimen
People
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Zaccharias Janssen invented compound microscope, uses multiple lenses
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Staining
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Prep
Heat fixing sticks organism to slide, kills it, prevents autolysis
Why?
Bacteria are colorless and small, stains make size and shape visible, and they give better contrast
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Differential stains
Gram stain
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Gram negative have inner and outer membrane, less peptidoglycan
Acid fast
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Ex. mycobacterium tb, mycobacterium leprae
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