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Microscopy - Coggle Diagram

- - - - Radiation = visible light
      - Lenses = glass
      - Max resolution = 200 nm
      - Specimen = living or dead
      - Image = colored (with stains)
  - - - Radiation = electrons
      - Lenses = electromagnets
      - Max resolution = 0.5 nm
      - Specimen = dead, dehydrated, in vacuum
      - Image = black & white (false color possible)
    - - TEM (Transmission EM) → internal cell structures
        SEM (Scanning EM) → cell surface, 3D image
- - - - Nucleus (chromatin + nucleolus)
      - Ribosomes (free + on RER)
      - Rough & Smooth ER
      - Golgi apparatus
      - Mitochondria
      - Lysosomes
      - Centrioles + microtubules (unique to animal cells)
      - Cell surface membrane
  - - - All organelles above (except centrioles)
      - Cell wall (cellulose)
      - Chloroplasts (plastids with chlorophyll)
      - Other plastids: chromoplast, leucoplast
      - Large central vacuole + tonoplast
      - Plasmodesmata
- - - - M = Magnification
      - I = Image size (measured with a ruler)
      - A = Actual size (real object size)