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Microscopy - Coggle Diagram
Microscopy
consist of
Resolution
Ability to distinguish two separate points
Light microscope: max resolution ±200 nm
Limited by the wavelength of visible light (400–700 nm)
Electron Microscopes
Electron wavelength is very short
high resolution (up to 0.5 nm)
TEM (Transmission)
views internal structures
SEM (Scanning)
views surfaces in 3D
Magnification & Measurement
Formula
M = I / A
Eyepiece graticule
scale in the eyepiece
Stage micrometer
tiny ruler for calibration
Comparison
Comparison (Light vs Electron Microscope)
Light microscope
uses light, glass lenses, can observe living cells, low resolution (200 nm)
Electron microscope
uses electrons, electromagnetic lenses, only dead cells, high resolution (0.5 nm), black-and-white images