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Microscopy - Coggle Diagram
Microscopy
History
Van Leeuwenhoek-1st microscope 300
Zaccharias Janssen-1st compound microscope
Joseph Jackson Lister- 1st modern microscope
Hans Christian Gram- created Gram stain
endospore
Bacteria survival bunker
when conditions bad-spore forms to protect DNA
When conditions improve- spore grows back into bacteria
Clostridium and Bacillus genera
staining
types
Simple stain-one dye
Baisic-positively charged ->attracted to negative cell wall
acidic- negatively charged -> repelled by negative cell wall
differential stain
Endospore stain- see inside cell see the cell spore
acid fast stain- distinguish if it has mycolic acid or no diagnostic stain
Gram stain-distinguish between Gram+ and Gram-
why
because bacteria are colorless
because better refractive index
measure of light binding activity
HEAT FIXING!
How to stain
gram stain-crystal violet->iodine->alcohol->safranin
acid base-carbol fuschin->acid alcohol->methylene blue
endospore stain-malachite green->water->safranin red