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Microscope - spectrum used - Coggle Diagram
Microscope - spectrum used
Light
convensional
bright field
bg white; image dark
dark field (light field)
bg dark; image white
Eg: ZEISS Lightfield 4D
uses 27 micro array lens to capture multiple images simultaneously and instantly reconstruct into 3D volume
pros is no delay in image taking, no stacking of images
since the capturing is instant, tracking of any signal has made possible, solving 4D imaging of dynamic biological processes (3D volume + time)
phase-constrast
differential interference contrast (DIC)
modified
fluorescent
bg dark; image coloured (from colours stained on samples)
confocal laser scanning
Eg: Sony SP8
inverted
polarized
super-resolution
Eg: ZEISS lattice SIM
uses lattice diffraction grid instead of traditional line grid
Electron
TEM
SEM
an important concept of how microscope works: CONTRAST
phase contrast
amplitude contrast
others
digital
scanning probes
https://www.slideshare.net/slideshow/types-of-microscope-250115126/250115126#2