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MICROSCOPY - Coggle Diagram
MICROSCOPY
Introduction
Microorganisms are very small:
- Bacteria: 0.2-1.5 µm wide, 3-5 µm long
- Viruses: 20-300 nm
- Fungi: Hyphae 2-10 µm; yeast 4-8 µm
- Parasites: Protozoa in µm; helminths up to meters
Most bacteria, fungi, parasites
light microscope; viruses
⬇
electron microscope
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Types of Microscopes
E. Electron Microscope
- Uses electron beams ➥ much higher resolution (0.5 nm)
- Types:
- TEM - Internal structures; specimen ultra-thin, fixed, dehydrated, embedded, sliced
- SEM - Surface details
- Uses: Viruses, fine ultrastructure, immune-EM
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B. Dark-field Microscope
- Bright specimen on dark background
- Special condenser blocks direct light; only reflected light enters objective
- Use: Thin, live, unstained bacteria (e.g., spirochetes)
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