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RRS- Extended Essay Biology - Coggle Diagram
RRS- Extended Essay Biology
Experimentation and Learning
Doing a trial experiment
This was to understand the possible challenges that I may encounter during the actual experiments, which would provide me with the knowledge on how to combat the issue.
Learning about the standard glucose curve
Learning how to use a spectrophotometer
Learning how to safely handle the apparatus
Learning about safe sterilization and incubation at safe temperatures
Understanding the pros and cons of my experiment conditions
Pros
Materials are easy to source
Experiment very easy to conduct
Cons
Incubation time due to the low temperature
Processing of the data
Learning about my dietary fibers
Learning about their chemical composition
Learning how to use the centrifuge at safe speeds without damaging the apparatus
Planning
Keeping all apparatus in an autoclave
Sourcing all my materials from the local godown
This includes all my dietary fibers
Preparation of solutions
1% maltose
Preparation of Yeast
1, 2 and 3% Concentrations
Rolled oats
Barnyard millet
Brown rice
Sorghum(Jowar)
Enzyme
Research
Dietary Fibers
Glycemic Index
Foods with varying glycemic index
Impact on enzymatic activity
Fiber interference
Procedure research
Using websites such as ScienceDirect for research articles and papers
Finding out alternatives to enzyme maltase, for exogenous addition
Principle of centrifuging and spectrophotometric analysis
SWOT analysis(evaluation of strengths and limitations
Strengths
My Supervisor
Sourcing of my dietary fibers
Can be sourced from local markets
Experimental setup
Preparation of solutions for incubation
Challenges
Understanding the results
This involves the analysis of the spectral data
Collection of data and plotting on standard glucose curve
Statistical tests
Oppurtunities
Research articles
Online calculators and softwares
For statistical tests
This involves tests such as the ANOVA and Tukey's test using the GraphPad PRISM 10.6.1
Using online calculators for mean, STDEV etc.
Experimentation trials
Threats
Experiment failing/not working
Invalid spectral data
With relation to absorbance
Contamination risks