Please enable JavaScript.
Coggle requires JavaScript to display documents.
BIOCHEMICAL TESTS, POSITIVE, BARITTS A-a-naphthol Acts as catalyst and…
BIOCHEMICAL TESTS
INDOLE :
MEDIA
Peptone broth,trptone broth,SIM agar
PRINCIPLE
TRYPTOPHAN->Tryptophanase->INDOLE+PYRUVICACID+NH3
INDOLE+KOVAC'S REAGENT------>Hcl--->ROSINDOLE+H2O
(Butanol) (cherry red)
-
-
VOGES PROSKAUER
-
PRINCIPLE
BUTANEDIOL FERMENTATION PATHWAY Alkaline end products
Glu+O2--->2,3 butanediol acetyl carbinol(acetoin)+co2+H20(ph 6.0)
AMC+a-naphthol->40%koHoxidation-->Diacetyl=Guanidine(pink complex)
RESULT
-
POSITIVE
PINK--RED
Enterobacter,klebsiella,staph,bacillus
-
CITRATE UTILIZATION
Principle
Citrate-->citrase-->oxaloacetic acid(Pyruvic acid)+Acetic acid+co2 co2+2Na+H2O --->Na2Co3(ph 7.6 and higher [blue]
-
Reagents
Bromothymol blue-Alkaline ph(Prussian blue)
Dipotassium phosphate-buffering agent
Magnesium sulphate-provides essential ions
Sodium chloride-maintains osmotic pressure
-
METHYL RED
-
-
PRINCIPLE
Mixed acid fermentation(acidic end products ) Glu+H20---Lac,acetic,formic acid+co2+H2(ph 4-4.4)
RESULT
POSITIVE
RED COLOUR
E.coli,klebsiella,shigella,salmonella,proteus,staph,lactococcus
-
-
POSITIVE
-
-
E.Coli,proteus vulgaris+_shigella dysentriae
-