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Material and methods - Coggle Diagram
Material and methods
ICC immunocytochemistry
- loai dong vat o primary antibody va loai o secondary co phu hop voi nhau khong
- what is nuclei counterstained with? -
Which fluorescence microscope used? For example: an upright fluorescence microscope (BX51, Olympus) with 20X and 40X objective lens and a laser Scanning Confocal Microscope (C1-Si, Nikon) with 40X objective lens.
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- Narrative a bit about each step and chemical used For example: wash with PBS and fixed with--- for ? time, permeabilized with 0,1% Trixon-100 and blocked with ? chemical and concentration used.
- next step: after blocking, which primary is used with which concentration / incubation with secondary antibody for 1 hour
Cells grown on coverslips were treated with ---- and fixed in----- for 15 min and cell were permeablized by 10 min incubation in ----, Primary antibody were incubated overnight at 4, Fluorescent Alexa-conjugated secondary antibodies were used91/...) and coverslips were mounted in ----. Imgae were captured on a ---
mention control: control sample, primary antibody, secondary antibody.
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The aim of ICC: we wil quantify from image to obtain both quantiative and qualitiative data for analysis.
MTT assay
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Finally, the compound were dissolved in DMSO
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The data analysis: the data obtained from the plate readers were uploaded and analyzed in Excel version ? and graphpad prism version ?
integrate, multiparameter analysis, including IC, standard curve, and the (listing the formula have used)
Based on the calculation, compounds were divided in two categories: high stressor (70% cell viability) and low stressor (90% cell viability)
To (decide) the (suitable) concentration to induce proteasomal stress, cell culture were treated with different concentration treatment ranging from 0nM to 200 nM,...
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research about: vehicle control (0,1 % DMSO ???)
Cell culture
SH-SY5Y
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what soes the role it play in investigating/ experiment? - reference. ( result it lead to, its credibility)
give a brief detail, where it's from.
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Material
obtained from ATCC
the environment ' what is the media , ( supplemented with 10% fetal bovine serum and penicillin/ streptomycin (pen/strep)
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Result interpretation:
MTT assay results
General trend remark
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To decide the treatment concentration suitable for the aim, low stressor (90% cell viability) and 70% (high stressor)