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Semen Evaluation & Preparation of Straws - Coggle Diagram
Semen Evaluation & Preparation of Straws
technique
Add antibiotics
Add criopreservative liquids if needed
Determine the sperm concentration, viability and motility using spectrophotometry or microscopy and a hemocytometer.
Inspect sample to ensure it is free of urine and other contaminants.
Dilute the samples in a 15 or 50 mL tube to 120 x 106 sperm/mL with 37 °C solution
Straws
produced by introducing a partitioning layer into a straw for artificial insemination to separate layers, thereby forming a semen storage layer containing an aqueous solution containing semen and an anti-freeze agent on one side and a dilution layer containing an aqueous solution made of tris citric acid, glucose and sodium chloride is used in the dilution
layer.
Semen Preservation
Refrigeration 15°C for max 48h
Freezing in semen tanks -196°C forever
Semen preparation for artificial insemination
After identifying the cane needed, bend the identification tab to a 45-degree angle to allow for easier removal of units.
Exact thawing procedures may be more or less critical based on the type of extender used in production of the units. However, as a general rule, straws should be thawed for a minimum of 35-37 °C for 40 seconds. Clean water should be used in the thaw bath. Before retrieving a straw from the tank, use a thermometer or a thaw card thermometer to confirm the water in the thaw bath is at
the correct temperature.
rember, fingertips are a source of heat, so it is critical to only touch the straw or straws you will remove from the cane for
thawing. It may be helpful to use forceps if removing only one straw for thawing or to avoid touching other straws.
Slide the plastic sheath over the straw and catheter. Most sheathes are designed with a tip in which the strawwill firmly “click” into place, preventing backflow
After loading the straw into the catheter, keep the loaded AI catheter warm and protected, either using an AI catheter warmer or tucking it into your shirt until it is time to AI. Although completely sterile technique is not necessary or possible, care should be taken to keep the sheath of the loaded catheter clean.
Semen preparation for artificial insemination
remove the straw from the thaw unit and dry it in a doubled-over paper towel as water is detrimental to sperm. insert the cotton plug end of the straw into the warmed AI catheter maintaining the paper towel around the catheter and straw
Using scissors or a straw cutter, cut approximately 0,5cm below the sealed end of the straw near the fluid line. Cut
the straw at an approximately 90-degree angle.
Load the catheter: pull the plunger of the warmed AI catheter back approximately 3 cm to allow for insertion of the straw into the warmed AI catheter.
Artificial insemination: heifer management
recommended that semen is deposited into a cow or heifer within 10 minutes of thawing, so be sure that the animal is already positioned for AI and work efficiently when a loaded catheter is prepared
technique
make sure that all equipment is cleaned and sanitary
Before putting on a shoulder-length sleeve, wrists and fingers should be free of any item that may be abrasive to the rectal lining of the cow or heifer.Most technicians choose to palpate with their non-dominant hand while manipulating the AI
catheter with their dominant hand. In addition, some technicians prefer to use an additional latex or nitrile glove on the outside of the sleeve to improve fingertip dexterity.
Apply non-spermicidal lubricant to the palpating hand