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Parasite detection and analysis in wildlife - Coggle Diagram
Parasite detection and analysis in wildlife
Sampling Types
Sample Source
Feces
likely
May include many other sample types and sources
Organ samples
Blood
Carcass
Methods
Invasive
Pros
Easier to collect and store samples
Less sampling bias
Cons
More paperwork
Increased chance of injury to staff and animal
Non-invasive
Pros
Easier to collect sample
Less likely to involve injury
Cons
You have to collect the sample in the field, preserve it in the field, and get it back to the facility
Sampling bias
Testing
Considerations
Sample preservation
Tissue Type
Time of exposure/ Since death
Parasite Analysis
Methods
Morphology
Includes
Micro- vs. Macroscopic
Larval Culture & Baermann Method
Tissue Compression & Lung Perfusion
DNA Sequencing
Includes
PCR
Sanger Sequencing
SNPs
mtDNA
DNA collection kits
CoproDNA (fecal)
Antibodies
Includes
ELISA
IFAT
Western Blot
Latex Agglutination
CATT (Card Agglutination Test)