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Diagnostic Principles 1st Agar: find…
Diagnostic Principles 1st Agar: find out what bacteria it is.
2nd Diagnostic Test: Find out the specifics of that bacteria
Agar
Selective Media
Buffered Charcoal Yeast Extract (BCYE)
Growth
Allows
Gram (-) Legionella Pneumophila (L-Cystine)
MOA
Added L-Cysteine (nutrient)
Added Activated Charcoal (decomposes H2O2)
Colonies
White Grey/blue
Tellurite Blood Agar
Growth
Allows
Corynebacterium
Prevents
Organisms that can not reduce potassium tellurite
Eosin Methylene Blue
Tellurite Blood Agar
MYC and SAB
Differential Media
Blood Agar (BAP)
Selective and Differential Media
Mannitol Salt Agar (MSA)
Growth
Prevents
Everything
Allows
Staph. A (Yellow) and Catalase Positve
Differential
Red to Yellow
Diagnostic: Can it eat Lactose + Sorbitol or not?
MacConkey (MAC)
Growth
Prevents
Gram +
Fastidious Gram (-)
Allows
Gram (-) that Ferment Lactose
Differential
Neutral Red to Bright Pink
Diagnostic
Can it eat Lactose or not?
Sorbitol MacConkey (SMAC)
Growth
Prevents
Allows
Gram (-) that ferment sorbitol
Differential
Neutral Red to Bright Pink
Gram (-) that do not ferment sorbitol are colorless
Hektoen Agar
Growth
Prevents
Gram (+)
Allows
Shigella
Salmonella
Differential
Green colonies = Shigella
Green with Black Centers = Salmonella (H2S) - non-lactose producing
Salmon Colored = Lactose Fermenters
Eosin Methylene Blue (selective only too)
Growth
Allows
Coliforms
Gram (-)
Prevents
Gram(+)
ENT/BEA
Growth
Prevents
Gram (+) Staph. (Bile)
Gram (-) (sodium azide)
Allows
Organisms that hydrolyze Esculin while in bile
Differential
Black = organisms that metabolize esculin which produces Esculetin. Esculetin reacts with ferric citrate making black
Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS)
Growth
Prevents
Enterobacteria
Enterococci
Gram (+)
Alows
Vibrio Cholerae
Enteropathogenic Vibrios
Differential
Yellow = Vibrio Cholera
Blue = Other Vibrios
Black centers in blue colonies = non-cholera vibrios (producing H2S
Enrichment Media
Chocolate (CHOC) Agar
Diagnostic Test