Please enable JavaScript.
Coggle requires JavaScript to display documents.
Lab methods course - Coggle Diagram
Lab methods course
Preparation
Read the script
Script layout:
Background
Cloning strategy
Aim (targets)
Protein-protein interaction analysis
functional analysis - subcellular localization
Appendix
Look up all the potential Protein-Protein
interactions of PCNA
Prepare methods for each day
What are we doing
Express other genes
in a human organism
Isolate & collect the potential
interaction partners
Immunoprecipitation
Western blot
Express PCNA
in model organism
tissues
human = mouse cell
plant = tobaco
other = nematode
PCNA-GFP + other molecular markers
examine sub-cellular localization