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Kirby-Bauer Method - Coggle Diagram
Kirby-Bauer Method
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Principles
diffusion of antimicrobial agents from a paper disc into an agar medium that has been inoculated with the test microorganism
antimicrobial agents diffuse outwards from the disk, creating a concentration gradient that inhibits the growth of the microorganism in its vicinity
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growth occurs when the bacteria reach a critical mass and can overpower the inhibitory effect of the antimicrobial compound
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Applications
clinical
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used to test wide range of bacterial infections including urinary tract infections, respiratory tract infections, and skin and soft tissue infections
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industrial
quality control of various industrial products including food, cosmetifcs, and pharmaceuticals
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research
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used to determine the minimum inhibitory concentration (MIC) of various antimicrobial agents required to inhibit the growth of bacteria
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Advantages & Limitations
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limitations
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organism variability
variability in growth can affect the size of the zone of inhibition, leading to potential errors