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The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel…
The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener
Introduction
Hydrogen sulfide
the toxic gas dubbed ‘gas of rotten eggs
however, is the fact that H2S is also a biological gas
H2S, at physiological concentrations, facilitated the induction of hippocampal long‐term potentiation
H2S relaxed rat aortic tissues in vitro
However, the cellular mechanisms for this vascular effect of H2S, as well as its physiological significance, remained to be examined
Results
The cardiovascular effects of H2S in vivo
An intravenous bolus injection of H2S at 2.8 and 14 μmol/kg body weight
bolus intravenous injection of a KATP channel opener pinacidil
bolus intravenous or intraperitoneal injection of glibenclamide (a KATP channel blocker)
Characterization of the H2S‐induced vasorelaxation
elaxation of rat aortic rings
Pretreatment of the endothelium‐intact tissues with l‐NAME
co‐application of charybdotoxin (50 nM) and apamin
Treatment of tissues with indomethacin
Involvement of K+ channel activities in the H2S‐induced vasorelaxation
Different relaxation potencies of H2S on vascular tissues precontracted by high or low concentration of KC
Involvement of KCa channels in the vascular effects of H2S
aortic rings were incubated with either 10 mM tetraethylammonium， 100 nM charybdotoxin or 100 nM iberiotoxin (IbTX) (two specific KCa channel inhibitors) for 20 min prior to the application of H2S
Involvement of voltage‐dependent K + (Kv) channels in the vascular effects of H2S
Involvement of KATP channels in the vascular effects of H2S
The direct effect of H2S on KATP channel currents and membrane potential in single vascular SMCs
After exposure to 300 μM H2S, KATP channel currents in rat aortic SMCs were significantly increased in amplitude
he effects of H2S and pinacidil on KATP channel currents were observed in a wide test potential range
仅使用阻断剂，可能由于年代原因没有从基因层面修改