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Selectivity of commonly used pharmacological inhibitors for cystathionine…

- - - - derived from the bacterial breakdown of organic matter that occurs in swamps and sewers under anoxic conditions
        
        H2S production is not restricted to bacteria, but higher eukaryotes, including humans, produce small amounts of H2S and use it as a signalling molecule
  - - - cystathionine β synthase (CBS)
        
        CSE and CBS catalyse several H2S-generating reactions using cysteine and/or homocysteine as substrates
      - cystathionine γ lyase (CSE)
      - third pathway that catalyses the production of H2S from L-cys via the combined action of 3-mercaptopyruvate sulfurtransferase
- - - - Recombinant proteins were then purified from bacterial lysates by affinity chromatography on GSH columns
        
        SDS-PAGE
        
        UV absorption spectra of CBS
    - - all subsequent experiments were performed using this amount of protein.
        
        the amount of PLP, a cofactor for both CBS and CSE that is needed for H2S generation
  - - - BCA is a more potent CSE inhibitor than PAG with IC50 of 14 and 40 μM respectively
        
        Subsequently, the ability of the above-mentioned inhibitors on CBS was tested
  - - - used rat aortic rings that are devoid of CBS expression