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Photosynthesis, Microbiology, Respiration, Populations and Ecosystems,…
Photosynthesis
Light Dependant stage
Transducers: convert light energy of photons hitting the leaf to chemical energy in the form biomass
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Light hits antenna complex and excites electrons of accessory pigments. This energy is transferred to the e- of chlorophyll A in the reaction centre which is excited to the next energy level and travels to an electron carrier. As it travels between carriers its goes down an energy level with the energy providing power to a proton pump. h+ ions are pumped into the stroma then travel down a stalked particle via chemiosmosis to generate ATP (ADP + pi + ATP synthetase)
e- and h+ used in the ETC are provided by the photolysis of water (h2o + light = h+, e- & 1/2O2)
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e- from PSII leaves the ETC and is accepted by NADP to form reduced NADP (e- replaced by photolysis of water)
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DCPIP experiment
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Solution of sucrose, DCPIP and chloroplasts should turn blue to green in the light
Adaptations
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Cells
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Cylindrical and at right angle to leaf surface to maximise no. cells and minimise number of cell walls light has to travel to to reach chloroplasts
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Microbiology
Aseptic technique
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Wipe desk, wash hands, use sterilised equipment, flame inoculating loops and necks of bottles etc... to avoid contamination of people by bacteria or of sample by contaminated equipment or people.
Autoclave
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Plastic equipment autoclaved in autoclave bags, marked as hazardous then thrown away
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Petri dish partially sealed with tape to avoid contamination but also anaerobic conditions that encourage parthenogenic bacteria
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Agar
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Streak plate: bacteria on loop spread in one direction then bacteria on plate spread 3 more times until bacteria are sparse enough to see
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Counting
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Serial Dilutions
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I part bacterial suspension + 1 part water dilutes by a factor of 10; repeat until optimum dilutation factor
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Spread dilution onto agar and allow to incubate; each resulting colony will have grown from a single bacteria; multiply count by dilution factor to find no. bacteria in original sample
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Gram Negative Bacteria
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Lipopolysaccharide prevents crystal stain from binding so are counter stained with safranin and appear red
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Cells wall is then structurally weak so water uptake via osmosis causes the cell to swell and burst (osmotic lysis)
antibiotics for gram-positive bacteria do not harm eukaryoic cells as they do not have peptidoglycan
Antibiotics for gram-negative bacteria interfere with protein synthesis and do not effect eukaryotic cells as they have different cellular machinery for
Respiration
ATP
Universal Energy Currency: used by all cells in all living things for all reactions requiring energy
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Adenine base, ribose sugar + 3 phosphate groups
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Active Transport, Exocytosis,
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Glycolysis
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Happens in cytoplasm as glucose is too big to fit through outer mitochondrial membrane and it is water soluable
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Phosphorylated glucose is polar so less likely to diffuse out of the cell and is more reactive so less activation energy required for enzyme reactions
Anaerobic Respiration
No oxygen so e- and h+ are not accepted after the ETC so NADH and FADH are not oxidised, no electrochemical gradient produce and no ATP synthesised. NAD and FAD are not regenerated so cannot accept h+ from the dehydrogenation of intermediated in the Krebs, link or glycolysis stage so all reactions cease. Pyruvate accepts h+ to regenerate NAD so glycolysis can continue and some ATP can still be made.
pyruvate accepts h+ from NADH so that NAD freed up to dehydrogenase triose phosphate so glycolysis can continue and ATP can still be produced
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Electron Transport Chain
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each NAD = 3ATP, each FAD = 2ATP
Efficiency
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ATP used to move pyruvate, NADH, FADH and ADP across the mitochondrial membrane
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Krebs Cycle
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Net = 6 NADH, 2 FADH + 1ATP
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Cyanide
Inhibits last electron carrier preventing e- and h+ from being transferred to water causing the electrochemical gradient to break down so no chemiosmosis or ATP synthesis leading to cell death
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