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Gram stain (Biochemical test identification) - Coggle Diagram
Gram stain
(Biochemical test identification)
Gram positive
Catalase test
Bubble formation
(Positive)
Staphylococcus sp
No bubble formation
(Negative)
Streptococcus sp
Colony Morphology
Staphylococcus sp
Manntinol Salt
Agar
Shape: Circular or round
Margin: Entire/ Irregular
Surface: Smooth, shiny
Elevation: Convex
Blood Agar
Shape: Circular or round
Margin: Irregular/Entire
Surface: Smooth, shiny
Elevation: Flat/Convex
Streptococcus sp
Manntinol Salt
Agar
No growth
Blood Agar
Shape: Circular or round
Margin: Undulating/ Entire
Surface: Smooth, shiny
Elevation: Low/ Flat
Coagulase test
Plasma coagulates
(Positive)
Staphylococcus sp
Plasma doesn't
coagulate
(Negative)
Streptococcus sp
Agar used
Blood agar
Manntinol salt agar
Gram negative
Agar used
MacConkey agar
Escherichia coli
Citrate test
Negative
Triple Sugar Iron
Test (TSI)
Yellow slant and butt
Glucose, lactose and sucrose fermenter
Acidic
H2S absence
Methyl Red
Test (MR)
Positive
Voges-Proskauer
Test (VP)
Negative
Indole Test
Positive
Oxidase Test
Negative
Colony morphology
Shape = irregular
Margin = undulated
Surface = Smooth and shiny
Elevation = Flat
Flat, dry, pink, non-mucoid conies with a surrounding darker pink area of precipitated bile salts.
Pink-red colonies on MacConkey medium indicate presence of lactose fermenter.
Pseudomonas aeruginosa
Citrate test
Positive
Triple Sugar Iron
Test (TSI)
Red slant and butt
Non glucose, lactose and sucrose fermenter.
Alkaline
H2S absence
Methyl Red
Test (MR)
Negative
Voges-Proskauer
Test (VP)
Negative
Indole Test
Negative
Oxidase Test
Positive
Colony Morphology
Shape = irregular
Margin = undulated
Surface = smooth and shiny
Elevation = Raised