Please enable JavaScript.
Coggle requires JavaScript to display documents.
First continuous marine sponge cell line established, M199 Medium, Added…
First continuous marine sponge cell
line established
Sponges
Geodia
barretti
Cells from 3 individuals
Sponge-derived microbes
Source of approximately 30% of marine natural products discovered yearly
OpM1 Medium
Cell culture
Results
Increased growth rate and higher maximum cell density in OpM1 than in M1
Associated with added components, namely, GF1 and Vit 1
Continuous marine sponge cell line
Possible development of production strain
Steady source of useful metabolites and other biomolecules from sponges
Up to at least 98 population doublings in OpM1 vs 5 doublings in M1
Possibly immortal due to maintained telomerase activity in sponges
M199 Medium
M1 Medium
Sponge-specific medium
Used for primary sponge cell cultures
Added components
L-glutamine, GF1, TE Vit1, PFT α, PDGF, RPMI, FBS, ITS-X, LM-1