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Ecosystem in Oral Cavity, ● - Coggle Diagram
Ecosystem in Oral Cavity
Classification prokaryote cell
Bacteria
Lack nucleus & organelles
Various shapes
Cell walls with peptidoglycan
Archaea with Eukarya
Similar to bacteria
Lack peptidoglycan
Objective
Identify
Categorization
Factors
Cell Morphology, Cellular Arrangement, Cell Envelope, Genetic Material, Microbial Community Composition, Metabolic Pathways, Environmental Factors.
Phenotyping & Genotyping Microorganisms
Phenotyping
Observable traits assessing factors the ability to form biofilm
Investigate fungal strains' biotechnological potential
Procedure
Collect fungal samples
DNA extraction
Amplification
Genotyping:
Data analysis
Interpretation and reporting
Genotyping
study of a virus's genetic structure/genetic composition associated with certain phenotypes
Identifying strains, shifting of microbial profiles in the progression of dental caries.
Example : Patients with severe periodontitis
Procedure
Collection of oral samples (e.g., saliva, plaque).
DNA extraction using appropriate protocols.
Library preparation for NGS.
Sequencing of microbial DNA.
Bioinformatic analysis to identify genetic variations and relationships.
Understanding evolutionary relationships.
Detection and identification of microorganism
Importance
Diagnosis, treatment, prevention
Distinguish strains
Example : Identifying periodontitis
by microbiological tests. Samples of plaque or tartar.
By polymerase chain reaction (PCR)
Objective
Identifying, Patient Management, Prevention of Spread, Environmental Monitoring, Prognostic Information, Optimization of Antibiotic Use
Detection
Diagnosis of Infections, Treatment Guidance, Prevention and Control, Research and Development
Rania Gisella F.H (2306172464
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