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Chromatography - Coggle Diagram
Chromatography
Different types
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Column Chromatography
This technique is used for the purification of biomolecules. On a column (stationary phase) firstly the sample to be separated
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The samples are accumulated at the bottom of the device in a tme-, and volume-dependent manner
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Affinity chromatography
this chromatography technique is used for the purification of enzymes, hormones, antibodies, nucleic acids, and specific proteins
A ligand which can make a complex with specific protein (dextran, polyacrylamide, cellulose etc) binds the filling material of the column.
The specific protein which makes a complex with the ligand is attached to the solid support (matrix), and retained in the column, while free proteins leave the column.
Then the bound protein leaves the column by means of changing its ionic strength through alteration of pH or addition of a salt solution
Paper chromatography
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In this method a thick filter paper comprised the support, and water drops settled in its pores made up the stationary “liquid phase.”
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Gas chromatography
stationary phase is a column which is placed in the device, and contains a liquid stationary phase which is adsorbed onto the surface of an inert solid. Gas chromatography is a “gas-liquid” chromatography.
ts carrier phase consists of gases as He or N2. Mobile phase which is an inert gas is passed through a column under high pressure.
The sample to be analyzed is vaporized, and enters into a gaseous mobile phase phase. The components contained in the sample are dispersed between mobile phase, and stationary phase on the solid support.
Gas chromatography is a simple, multifaceted, highly sensitive, and rapidly applied technique for the extremely excellent separation of very minute molecules.
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Applications
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Quality control
uality control. Manufacturing relies on gas chromatography for quality control, companies that produce cars, chemicals, and pharmaceuticals, in particular
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Food industry
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Food and Beverage. Quality control within the food and beverage industry can be enacted through chromatography
Forensic science
application of science principles and methods to support legal decision-making in matters of criminal and civil law
During criminal investigation in particular, it is governed by the legal standards of admissible evidence and criminal procedure.
Food analysis
Food analysis and quality control are one of the most common applications for gas chromatography due to their component quantification, accuracy, and speedy
Gas chromatography is vital to the food industry for ensuring the safety of food products, preventing contaminated products
What is Chromatography?
Allows the separation, identification and determination of chemical components in complex mixtures.
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Chromatographies are processes that encompass several separation techniques, based on physical properties of certain materials
In interaction with substances or mixtures of substances, which are related to their chemical properties, allow the decomposition of a mixture and analyze its constituents.
With the development of technology, chromatographic techniques diversified and significantly improved their ability to resolve mixtures of different natures