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technology route - Coggle Diagram
technology route
animals randomized
CUMS treat
normal treat
Immunofluorescence
label NMDA receptor and CREB protein in BLA
Comparing the expression differences of NMDA receptors and CREB in BLA between cums and normal treating
Whether NMDA receptors and CREB in the BLA involved in the generation of depressive states
animals randomized
CUMS treating
Western Blot
quantitative measures NMDA recepto and CREB proteins in BLA
quantitative measures BDNF and CRH in hypothalamus
Comparing the expression differences of BDNF and CRH in hypothalamus between cums and normal treating
Whether CRH that is the key of HPA and BDNF involved in the generation of depressive state
Normal treating
animals randomized
CUMS treating
Microdialysis
comparing the Neurotransmitters in hypothalamus between cums and normal treating
Differences in hypothalamic neuron and neurotransmitter activity during depression
tissues extract
frozen section
site identification
Normal treating
animals randomized
Normal treating
CUMS treating
ArcLight Optogenetics
comparing the neuronal activity in hypothalamus between cums and normal treating
animals randomized
CUMS treating + NMDA receptor activity enhanced
Microdialysis
comparing the Neurotransmitters in hypothalamus between cums and NMDA receptor enhanced after CUMS
To explore whether NMDA receptors in the BLA regulate the hypothalamus during depression，and whether change depression after NMDA receptor enhanced in BLA
animals randomized
CUMS treating + NMDA receptor activity enhanced
ArcLight Optogenetics
comparing the neuronal activity in hypothalamus between cums and NMDA receptor enhanced after CUMS
animals randomized
CUMS treating + NMDA receptor activity enhanced
Western Blot
quantitative measures BDNF and CRH in hypothalamus
animals randomized
CUMS treating + CREB blockade
Microdialysis
comparing the Neurotransmitters in hypothalamus between cums and CREB inhibited after CUMS
To explore whether the NMDA receptor-CREB pathway in the BLA regulates the hypothalamus during depression，and whether change depression after
CREB blockade in BLA
animals randomized
CUMS treating + CREB blockade
ArcLight Optogenetics
comparing the neuronal activity in hypothalamus between cums and CREB inhibited after CUMS
animals randomized
CUMS treating + CREB blockade
Western Blot
quantitative measures BDNF and CRH in hypothalamus
animals randomized
CUMS treating + NMDA receptor activity enhanced
forced swim test
Observe changes in the emotional state of test animals after NMDA receptor enhanced
sucrose preference test
animals randomized
CUMS treating + CREB blockade
forced swim test
Observe changes in the emotional state of test animals after CREB blockade
sucrose preference test