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Lab 5- Thin layer chromatography - Coggle Diagram
Lab 5- Thin layer chromatography
Label the 6 test tubes
Caffeine Standard
Anacin
Acetaminophen
Excedrin
Aspirin
Caffeine Product
add 3-4 drops of each solution in test tubes
Add one capillary tube to each test tube
scrap small amount of caffeine with tip of spatula
put caffeine in appropriate test tube
Avoid touching coated surface of TLC plate
hold plate by sides
obtain 2 TLC plates
One for standards and one for test solutions
6 spots on silica plate
1 more item...
after developing plates, calculate and record Rf values for each spot
identify components in each mixed analgesic by Rf values
add 1-2 mL of ethanol to that test tube and mix suspension well
use vortex to mix if necessary