Cryptosporidium parvum

Major cause in cattle

1907 Tyzer first reported from mouse
Hymen infected in 1976

Opportunistic pathogen for immuno compromised patient and cause secondary infection

Habitat

Epithelial layer of intestine
C.felis, c. canis, c.muris- mammals

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Morphology

Size 4- 5 um in diameter

Spherical or oval

Cyst covered by double wall

4 present shape sporozoites

Anterior pointed posterior broader

One nucleus

Only cyst form

Satined with acid fast

Life cycle

Single host

Two cycle

Sexual

Asexual

Schizogony

Gametogony

Ingestion of contaminated food

First attach on tissue

Cyst wall broken by gastric juice and release sporozoites

Attach on intestinal epithelial

Undergoes excystation

Multiply and develop type 1 meront contain 8 merozoite

Either again affect intestinal epithelial layer and form type 2 meront

Release 4 merozoites

Form type 2 merozoites

Micro gamete

Macrogamete

Release 12- 16 microgamete

Release one macrogamete

Zygote form of oocyst

Thick walled

Thin walled

Unsporated more effective

Autoinfection again infect intestine

Excrete through feces

Pathogenicity

Opportunistic pathogen

Immunocompetent develop self limiting, diarrhea

Immuno compromised patients in severe case life threatening cholera like illeness

Lab diagnosis

Direct wet mount

Acid fast staining

Immunoflurescent antibody

Histopathology cal examination

Haematoxylin and eosin stain

Treatment

Immuno competent host

Treatment to prevent dehydration

AIDs or congenital deficiency

Continuous supportive therapy

Antimicrobial agents

Azithromyzin, paromycin, spiramycin, octreotide and nitazoxanic