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Types Of Culture Media - Coggle Diagram
Types Of Culture Media
1) Basic medium:
Typically composed of peptones, extracts, salts, and water.
It is a medium containing the basic nutrients for the growth and reproduction of general microorganisms.
Examples:
Nutrient Agar:
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Composition: 0.5% Peptone,0.3% beef extract/yeast extract,0.5% agar,0.5% NaCl, Distilled water.
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Tryptic Soy Broth (TSB).
It is used for confirmation of Campylobacter jejuni by motility test. It is suitable for the cultivation of both aerobic and anaerobic bacteria.
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Composition: Peptone, Soybean Digest, Sodium Chloride, Dextrose (Glucose),Water.
2.Enrichment medium:
It may contain blood, serum, vitamins, or other growth factors.
Examples:
Blood Agar:
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It is particularly useful for the isolation of Streptococcus species, Staphylococcus aureus, and other hemolytic bacteria
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Chocolate Agar:
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It is used for culture of pneumococcus, gonococcus, meningo- coccus and Haemophilus.
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3.Selective medium.
Examples:
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MacConkey Agar
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Composition: This medium include crystal violet dye, bile salts, lactose, and neutral red (pH indicator).
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Mannitol Salt Agar
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It is used for isolating pathogenic staphylococci from clinical samples, food and other materials of sanitary importance.
Composition: It is consist of peptones, mannitol, sodium chloride, phenol red indicator, agar, and water.
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Composition: Selective agents such as antibiotics, dyes, or chemicals are added to inhibit the growth of unwanted organisms.
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4.Identification medium.
It is to add some reagents or chemicals into the culture medium, so that some changes will occur after culture,
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Composition: Besides basal components, indicator media contain pH indicators or other chemical indicators.
Example
Phenol Red Broth
Role: prepared with phenol red as an indicator of acid production. A color change from red- orange to yellow occurs when acid is produced as a result of carbohydrate metabolism.
Composition: It consist of dextrose Proteose peptone and beef extract provide nitrogenous nutrients to the organisms.
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