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Selective & Differential media - Coggle Diagram
Selective & Differential media
Lab 1 (Water testing)
m-Endo agar
selective: sodium sulfite + basic fuchsin (inhibit gram +)
differential: lactose (fermenters show as pink colonies)
E.coli on m-endo
E. faecalis = pink to rose/red
m- Enterococcus agar
selectivity: sodium azide (inhibits gram -)
indicator: Triphenyl tetrazolium chloride (TTC)
E. faecalis on m- Enterococcus
LSTB tubes
selective: sodium lauryl sulfate (inhibits non-coliforms)
differential: lactose
positive result: turbidity and bubble on durham tube
BHI agar (Brain Heart Infusion)
light amber/beige
general purpose media
6.5% NaCl= selective for high salt tolerance bacteria
enterococci grow
BGLB tubes
selective: Ox-bile and Brilliant green inhibit gram +
differential: lactose
BEA slant
selective: bile inhibits gram + bacteria
entercocci gorw
differential: esculin + ferric citrate
blackening of media is positive
M-FC agar
selective: Bile Salts (inhibits gram +)
differential: aniline blue and rosolic acid, lactose
E.coli on MF-C
EMB agar
differential: lactose
selective: eosin Y and methylene blue dyes (inhibit gram+)
E.coli on EMB
sucrose promotes growth of coliforms
EC broth
selective: Bile salts inhibit gram +
differential: lactose
