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LEISHMANIA, Life cycle, Lab diagnosis
Sample: blood
Microscopic…
LEISHMANIA
H
Morphology
2 forms
Promostigate
Found in midgut of sand fly
Spindle shape(15-25 mm long)
Carry single flagellum,15-30mm long
Have nucleus,axoneme,basal body
Kinetoplast
No undulating membranes sometimes it have
Amostigate
Oval shape
2-4mm in length
Non motile,1 nucleus,
Food vacuoles,bleopharaplasand axoneme
Found within reticuloendothelial system
Pathogenesis
Pathogenesis
Dum dum fever
Splenomegaly, anemia
Lympadenopathy
Hepatomegaly
Fever, diarrhoea,
Vommitig etc .
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Epidermology
Leishmaniasis endemic in 88
Countries like africa,south america
Asia, Europe,north America
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Lab diagnosis
Sample: blood
Microscopic examination
Staining: geimsa stain
Culture:NNN MEDIUM
Novy NvC Nicolle
SERIOLOGICALtest
ELISA,IFT,CIA,IDFA
molecular test:PCR
Confirmatory test:
Leishmanin
Montenegro test
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